FISH-metaphase: A technique used to identify the presence of specific chromosomes or chromosomal regions through
hybridization (attachment) of fluorescently-labeled DNA probes to denatured chromosomal DNA. Examination under fluorescent
lighting detects the presence of the hybridized fluorescent signal (and hence presence of the chromosome material) or absence
of the hybridized fluorescent signal (and hence absence of the chromosome material). With metaphase FISH, cells progress
through the division process until metaphase, when chromosomes are condensed and can be individually distinguished. In
contrast to interphase FISH, metaphase FISH permits visualization of the actual chromosomes as well as the general location of
the abnormality on the chromosome.
FISH Testing
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